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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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(a) Schematic of <t>the</t> <t>FGF21</t> gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by <t>ELISA.</t> (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).
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Image Search Results


(a) Schematic of the FGF21 gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by ELISA. (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).

Journal: bioRxiv

Article Title: Regulatable In Vivo Gene Expression via Adaptamers

doi: 10.64898/2025.12.21.695777

Figure Lengend Snippet: (a) Schematic of the FGF21 gene therapy strategy, where inducible FGF21 expression enhances fat metabolism, improves glucose tolerance, and reduces body weight in obese mice. (b) Serum FGF21 levels in diet-induced obese (DIO) C57BL/6 mice following injection of 2×10¹¹ vg per animal, measured by ELISA. (c) Changes in body weight expressed as a proportion of initial weight at the time of AAV injection. (d) Glucose tolerance test performed on Day 22. (e) Hematoxylin–eosin staining of liver sections showing reversal of hepatic steatosis in FGF21 + tetracycline–treated mice. Error bars represent mean ± s.e.m. (n = 4).

Article Snippet: Serum FGF21 was quantified using Mouse FGF21 DuoSet ELISA kit (R&D DY3057) and an ELISA Starter Kit (Bethyl Laboratories E101) according to manufacturer protocols.

Techniques: Expressing, Injection, Enzyme-linked Immunosorbent Assay, Staining